Panel: TOPO Tools: Creating Linear Expression Constructs with Functional Elements227.Panel: BioBricks and the Ordered Assembly of DNA Fragments225.Panel: A Guide to Cloning the Products of Polymerase Chain Reactions218.Panel: The History of Transformation of Bacteria by DNA217.coli: Transformation and Storage of Bacterial Cells in the Same Solution175 Panel: Alternative Protocol: One-Step Preparation of Competent E.Panel: Cloning and Transformation with Plasmid Vectors157.Protocol 14: Screening Bacterial Colonies Using X-Gal and IPTG: -Complementation.Protocol 13: Cloning PCR Products: TOPO TA Cloning.Protocol 12: Cloning PCR Products: TA Cloning.Protocol 11: Cloning PCR Products: Making T Vectors.Protocol 10: Cloning PCR Products: Blunt-End Cloning.Protocol 9: Cloning PCR Products: Addition of Restriction Sites to the Termini of Amplified DNA.Protocol 8: Attaching Phosphorylated Adaptors/Linkers to Blunt-Ended DNAs.Protocol 7: Dephosphorylation of Plasmid DNA.Protocol 6: Cloning in Plasmid Vectors: Blunt-End Cloning.Protocol 5: Cloning in Plasmid Vectors: Directional Cloning.coli: Nanoparticle-Mediated Transformation Protocol 2: The Inoue Method for Preparation and Transformation of Competent E.Protocol 1: The Hanahan Method for Preparation and Transformation of Competent E.Chapter 3: Cloning and Transformation with Plasmid Vectors157.Panel: Formamide and Its Uses in Molecular Cloning153.Panel: Additional Protocol: Stripping Probes from Membranes150.Panel: Additional Protocol: Autoradiography of Alkaline Agarose Gels117.Protocol 13: Southern Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Membranes.Protocol 12: Southern Blotting: Simultaneous Transfer of DNA from an Agarose Gel to Two Membranes.Protocol 10: Isolation of DNA Fragments from Polyacrylamide Gels by the Crush and Soak Method.Protocol 9: Recovery of DNA from Low-Melting-Temperature Agarose Gels: Organic Extraction.Protocol 8: Recovery of DNA from Agarose Gels Using Glass Beads.Protocol 7: Imaging: Autoradiography and Phosphorimaging.Protocol 6: Alkaline Agarose Gel Electrophoresis.Protocol 5: Detection of DNA in Polyacrylamide Gels by Autoradiography.Protocol 4: Detection of DNA in Polyacrylamide Gels by Staining.Protocol 3: Polyacrylamide Gel Electrophoresis.Protocol 2: Detection of DNA in Agarose Gels by Staining.Protocol 1: Agarose Gel Electrophoresis.Panel: Minimizing Damage to Large DNA Molecules77.Panel: DNA Extraction from Formaldehyde-Fixed Tissue Embedded in Paraffin Blocks72.Panel: Alternative Protocol: One-Tube Isolation of DNA from Mouse Tails62.Panel: Alternative Protocol: Isolation of DNA from Mouse Tails without Extraction by Organic Solvents61.Panel: Commercial Kits for Purification of DNA3.Panel: Isolation and Quantification of DNA1.Protocol 19: Quantifying DNA in Solution with PicoGreen.Protocol 18: Estimating the Concentration of DNA by Fluorometry Using Hoechst 33258.Protocol 17: Using Ethidium Bromide to Estimate the Amount of DNA in Bands after Electrophoresis through Minigels.Protocol 16: Rapid Isolation of Yeast DNA.Protocol 15: Preparation of Genomic DNA from Mouse Tails and Other Small Samples.Protocol 14: A Single-Step Method for the Simultaneous Preparation of DNA, RNA, and Protein from Cells and Tissues.Protocol 13: Isolation of High-Molecular-Weight DNA from Mammalian Cells Using Proteinase K and Phenol.Protocol 12: Isolation of High-Molecular-Weight DNA Using Organic Solvents to Purify DNA.Protocol 11: Preparation of Double-Stranded (Replicative Form) Bacteriophage M13 DNA.Protocol 10: Growing Bacteriophage M13 in Liquid Culture.Protocol 8: Preparation of Single-Stranded Bacteriophage M13 DNA by Precipitation with Polyethylene Glycol. Protocol 7: Concentrating Nucleic Acids by Extraction with Butanol.Protocol 6: Concentrating and Desalting Nucleic Acids with Microconcentrators.Protocol 5: Precipitation of DNA with Isopropanol.Protocol 4: Precipitation of DNA with Ethanol.Protocol 3: Isolating DNA from Gram-Negative Bacteria (e.g., E.Protocol 2: Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreps.Protocol 1: Preparation of Plasmid DNA by Alkaline Lysis with SDS: Minipreps.Chapter 1: Isolation and Quantification of DNA1.Table of Contents Expand All | Contract All
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